AIM/HYPOTHESIS
A new batch of antibodies arrived that will be used for IPs, and hence needed to be conjugated to Dynabeads.
MATERIALS
| Name | Company | Cat. nr. | Date received/made | Comments | ||||
|---|---|---|---|---|---|---|---|---|
| Dynabeads M270 Epoxy | Invitrogen Dynal | 143.02D | ||||||
| a-ORF1p abs | Abmart | 5310-1-4/4H1 | received Nov 18 2019 | Custom synthesized, identical to the Milipore Sigma product: anti-ORF1p 4H1 |
METHODS
Conjugation was done according to Conjugation of antibody Dynabeads. Used volumes:
* 103ul antibody
* 100ul 3M ~AmSO4
* 97ul 0.1M ~NaPO4, pH 7.4RESULTS
Concentrations of antibody mix:
Before desalting 1.5 mg/ml
After desalting 1.45 mg/mlSee for validation Testing of new anti-ORF1p beads_Dec 4 2019
DISCUSSION
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