LaCava Research Wiki

Initiated September 2017

Angelica Ferguson's Journal

Angelica10th January 2020 at 11:13am

11/20/2019 Novus antibody titrations anti-TP53BP1 and anti-OBSL1

10/28/2019 Refeyn Report L1 RNP Mass Photometry

10/02/2019 ORF2/ORF1 Tandem IP for Electron Microscopy (EM)

09/18/2019 ORF2/ORF1 Tandem IP for Mass Photometry (+RNasin)

09/09/2019 ORF2/ORF1 Tandem IP with BS3 Crosslinking (gel protocol)

08/29/19 ORF2/ORF1 Tandem IP LD401 Native Elutions Tests

08/16/2019 Flow Cytometry sorting of -/- OBSL1 cell line

08/13/19 ORF2/ORF1 Tandem IP LD401 with BS3 crosslinking titration in solution

08/01/19 ORF2/ORF1 Tandem IP LD401 with BS3 crosslinking titration in solution

07/23/19 ORF2/ORF1 Tandem IP LD401 with BS3 crosslinking titration on beads

07/18/19 ORF2/ORF1 Tandem IP LD401 with BS3 crosslinking titration

07/03/2019 Anti-TP53 IPs from HEK293 Wild Type testing CDI antibodies

06/28/2019 Grid freezing for CryoEM with LINE-1 Tandem IP complexes

06/24/2019 ORF2/ORF1 Tandem IP with sucrose cushion for EM LD401

06/18/2019 CDI Antibody concentration and anti-TP53 bead conjugation

06/18/2019 Flow Cytometry sorting of -/-TP53BP1 cell line

05/30/2019 anti-TP53 IP FLpIn T-Rex vs HEK293 WT (ATCC CRL-1573)

05/29/2019 Orf2p/ORF1p Tandem IP (LD401 SILAC powder 4/24/14) for EM

05/23/2019 Conjugation of Dynabeads with anti-TP53 antibody

05/16/2019 Orf2p/ORF1p Tandem IP (LD401 SILAC powder 4/24/14)

05/13/2019 Orf2p/ORF1p Tandem IP (LD401 SILAC powders 4/24/14, 5/2/14)

04/16/2019 ORF2p anti-FLAG IP (LD401 heavy (04/24/14), heavy (08/27/12), heavy (05/02/14), light (05/02/14), LD222

04/15/2019 ORF2p/ORF1p Tandem IP (LD401 heavy powders 8/27/12, 5/2/14

04/10/2019 PVSA Testing for RNA IP - Tumor 162T and Control MT302 - Zymo w/ Phasemaker

04/08/2019 ORF2p anti-FLAG IP (LD401 heavy (04/24/14), heavy (08/27/12), heavy (05/02/14), light (05/02/14), LD222

04/03/2019 ORF2p anti-FLAG IP (LD401 heavy (04/24/14), heavy (08/27/12), heavy (05/02/14), light (05/02/14), LD222

03/26/2019 ORF2p/ORF1p tandem (LD401 heavy)

03/29/2019 ORF2p/ORF1p tandem (LD401 heavy)