LaCava Research Wiki

Initiated September 2017

August 2021 – Hua_ORF1 screen in tumors

admin9th January 2022 at 9:41am

Description: ORF1 screening in two different tumors. Two different controls were made: one using IgG IPS on tumor cells and other using anti ORF1 on normal cells.

Link to experiment and sample prep: https://macromolecule-child.rockefeller.edu/#July%202021%20-%20CRC%2024-well%20screen_%20162%20and%20197

Resuspension solvent: 94.9:5.0:0.1 water:MeOH:FA

Resuspension volume: 25 uL

Injection volume: 5 uL

MS method: uPU-60mingradTOP20-highload_Luciano_IP_gradient_45min_3_to_50_375_1500

Link to RAW files: https://www.dropbox.com/sh/fwwtr76hunab7f9/AAAXeg6aIYXB9jPablVUmjKga?dl=0