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CRYO - 2020 Sep 24 - RRP6 and LD401

admin3rd August 2021 at 1:20pm
Glow Discharge2 minutes vacuum, 50mA, 15 secondsBAD
Sample1RRP6 with RNase2020/Sep/23September 23, 2020 - RRP6 purification with RNase A/T1 treatment for EM_092320
1RRP6 300mM with RNAseVolume 2uL, Time 2.0sec, Blot Force 4White Holder
2RRP6 300mM with RNAseVolume 2uL, Time 1.5sec, Blot Force 4White Holder
3RRP6 300mM with RNAseVolume 2uL, Time 1.0sec, Blot Force 4White Holder
3RRP6 300mM with RNAseVolume 2uL, Time 2.0sec, Blot Force 2White Holder
Sample2LD401 Tandem IP for EM2020/Sep/16September 16, 2020 - LD401 Tandem IP for EM
1LINE-1 ORF1p 300mMVolume 2uL, Time 2.0sec, Blot Force 4Blue Holder
2LINE-1 ORF1p 300mMVolume 2uL, Time 2.0sec, Blot Force 2Blue Holder
3LINE-1 ORF1p 300mMVolume 2uL, Time 1.5sec, Blot Force 2Blue Holder
4LINE-1 ORF1p 300mMVolume 2uL, Time 1.5sec, Blot Force 2Blue Holder

The glow discharge was very bad. Effectively, grids were not glow discharged.
Samples are hydrophobic.