LaCava Research Wiki

Initiated September 2017

December 2020 - ROME screen_Nastya

jlacava6th January 2022 at 6:51am

Description: First ROME screen performed by Nastja, aiming to compare both 24 well plates and “by hand” methods

Link to experiment: N2102EP 24-well Hand Screen 11-12-2020

Link to sample prep: 22/12/2020_S-Trap Micro Ulra High Recovery Updated Protocol (Nastya's IP buffer screening, part 1)

29/12/2020_S-Trap Micro Ulra High Recovery Updated Protocol (Nastya's IP buffer screening, part 2)

Resuspension solvent: 94.9:5.0:0.1 water:MeOH:FA

Resuspension volume: 25 uL

Injection volume: 1 and 5 uL as indicated in the Raw file’s name

MS method: uPU-60mingradTOP20-highload_Luciano_IP_gradient_375_1500

Link to RAW files: https://www.dropbox.com/sh/tnb9szoue4gwwt4/AAAgU8A-HjNZWU5rD-Wpu1Kna?dl=0