LaCava Research Wiki

11/02/2020_RRP6 purification for mass spectrometry

13/03/2020_S-Trap Micro Ulra -High Recovery Protocol

27/03/2020_S-Trap Micro Ulra -High Recovery Analysis

05/05/2020_Presentation: Methods used in native mass spectrometry

15/05/2020_Presentation: Crosslinking mass spectrometry

12/06/2020_ Mass Photometry experiments (protein test samples)

xx/xx/xxxx_Cell Biology Samples Mass Spectrometry Analysis

16/06/2020_S-Trap Micro Ulra -High Recovery Protocol

26/06/2020_Presentation:R obust transcriptome-wide discovery of RNrnA-binding protein binding sites with enhanced CLIcliP (ecliP)

07/07/2020_ Mass Photometry_Aldolase_Cross_linking

10/09/2020_ Mass Photometry_Aldolase_Cross_linking_extra information

22/07/2020_S-Trap Micro Ulra -High Recovery Protocol (MgCl2)

24/07/2020_S-Trap Micro Ulra -High Recovery Protocol (NaCl 300/700 mM)

29/07/2020_Protein Mix for S-Trap training purposes

05/08/2020_S-Trap Micro Ulra -High Recovery Protocol (Marty Taylor_Whitehead Institute)

01/09/2020_S-Trap Micro Ulra -High Recovery Protocol (Nasty's IP samples, 300/700 mM NaCl and 100 mM MgCl)

04/09/2020_S-Trap Micro Ulra -High Recovery Protocol (Raghu Chivukula's LysoIP samples, RC9-132)

01/10/2020_Mass Photometry_Aldolase_Cross-linking (ethanol, urea, guanidine)

06/10/2020_ Mass Photometry (Aldolase crosslinking with BS3, reaction)

02/10/2020_S-Trap Micro Ulra -High Recovery Protocol (cross-linked Aldolase)

15/10/2020_Presentation MP progress

27/10/2020_Mass Photometry Aldolase stability test

03/11/2020_Mass Photometry Aldolase crosslinking with BS3 reaction part 2

05/11/2020_Nastya's SDS-PAGE protocol for dummies

13/11/2020_S-Trap Micro Protocol (Raghu Chivukula's LysoIP samples, RC10-30, changed protocol)

16/11/2020_Protocol Mass Photometry Experiments

17/12/2020_LCMS sample queue: Hua's N2102EP anti-ORF1 IP with Nuclease treatments

19/12/2020_LCMS sample queue: Hua's N2102EP L1 screen in 24-well format

22/12/2020_S-Trap Micro Ulra High Recovery Updated Protocol (Nastya's IP buffer screening, part 1)

23/12/2020_LCMS sample queue: Nasty's IP buffer screening part 1

29/12/2020_S-Trap Micro Ulra High Recovery Updated Protocol (Nastya's IP buffer screening, part 2)

29/12/2020_LCMS sample queue: Nasty's IP buffer screening part 2.

20/01/2021_SEPMAG LAB nano validation

19/02/2021 Exosome-10 IP (NaCl; 300 mM and 700 mM) for MP analysis

19/02/2021 Exosome-10 MP analysis (NaCl; 300 mM and 700 mM)

23/02/2021_SEPMAG LAB nano validation presentation

08/03/2021 Exosome-10 IP (NaCl; 300 mM and 700 mM) for MP analysis

08/03/2021 Exosome-10 MP analysis (300 mM and 700 mM NaCl IP)

10/03/2021 Mass Photometry (Exosome-10 IP analysis (NaCl; 300 mM and 700 mM)) IP buffer contamination

10/03/2021 Mass Photometry (Exosome-10 IP analysis (NaCl; 300 mM and 700 mM from 19/02/2021)) medium camera settings

22/03/2021 Exosome-10 IP (NaCl; 700 mM, 150 mg cell powder) for MP analysis

23/03/2021 Mass Photometry (Exosome-10 IP analysis (NaCl; 700 mM)(150 mg scale))

08/04/2021 Exosome-10 IP (NaCl; 300 mM, 200 mg cell powder) for MP analysis

08/04/2021 Mass Photometry (Exosome-10 IP analysis (NaCl; 300 mM)(200 mg scale))

13/04/2021 Bradford Protein Assay (standard 1 mL cuvettes)

17/04/2021_S-Trap Micro Protocol (Berezikov Group, Joscha Muck)

17/04/2021_LCMS sample queue: Berezikov group, Joscha Muck's samples

27/04/2021_Maxquant: Berezikov group, Joscha Muck's samples

13/05/2021 Exosome-10 IP (NaCl; 300 mM, 150 mg cell powder) for MP analysis

15/05/2021 MP Exosome tests (150 mg 300 mM NaCl IP Apostolis)

16/05/2021 Exosome-10 IP (NaCl; 300 mM, 150 mg cell powder) Bradford and SDS-PAGE

27/05/2021 Powerpoint: Berezikov group, Joscha Muck's samples

29/05/2021_S-Trap Micro Ulra High Recovery Protocol rev 5/27/21 (Rome IP buffer screening, part 1)

29/05/2021_LC-MS sample queue: Rome IP buffer screening, part 1

30/05/2021_S-Trap Micro Ulra High Recovery Protocol rev 5/27/21 (Rome IP buffer screening, part 2)

30/05/2021_LC-MS sample queue: Rome IP buffer screening, part 2

07/06/2021 Exosome-10 IP (NaCl; 300 mM, 150 mg cell powder, Nastya and Apostolis)

07/06/2021 MP Exosome-10 tests (NaCl 300 mM, 150 mg cell powder, Nastya and Apostolis)

08/06/2021 Exosome-10 IP (NaCl 300 mM, 150 mg cell powder, Nastya and Apostolis) SDS-PAGE

12/06/2021 Exosome-10 IP (NaCl; 300 mM, 150 mg cell powder, Nastya and Apostolis)

12/06/2021 MP Exosome-10 tests (NaCl 300 mM, 150 mg cell powder, Nastya and Apostolis)

15/06/2021 Exosome-10 IP (NaCl 300 mM, 200 mg cell powder, Nastya and Apostolis)

15/06/2021 MP Exosome-10 tests (NaCl; 300 mM, 150 mg cell powder IP, Nastya and Apostolis)

20/06/2021 Exosome-10 IP (NaCl; 300 mM, 150 mg cell powder, Nastya and Apostolis)

20/06/2021 MP Exosome tests (150 mg 300 mM NaCl IP Nastya and Apostolis)

20/06/2021 Native PAGE Exosome tests (150 mg 300 mM NaCl IP Nastya and Apostolis)

25/06/2021 Mass Photometry (Native Marker in "PBS with 1 % glycerol" and “NativePage sample buffer 1x”)

30/06/2021 Exosome-10 IP (NaCl; 300 mM, 150 mg cell powder, Nastya and Apostolis)

30/06/2021 Native PAGE Exosome tests (150 mg 300 mM NaCl IP Nastya and Apostolis)

15/07/2021_S-Trap Micro Ulra High Recovery Protocol rev 5/27/21 (Arthur Flohr Svendsen)

27/07/2021_S-Trap Micro Ulra High Recovery Protocol (300 mM NaCl IP samples, UPS2)

29/07/2021_S-Trap Micro Ultra-High Recovery Protocol rev. 05/27/21 (Berezikov Group, Stijn Mouton)

05/08/2021 anti-FLAG bead conjugation

09/08/2021 Exosome-10 IP (new anti-FLAG beads vs old anti-FLAG beads, NaCl; 300 mM, 150 mg cell powder) for MP/MS/Zipchip analysis

09/08/2021 Exosome-10 IP (new vs old anti-FLAG beads, NaCl; 300 mM, 150 mg cell powder) SDS-PAGE

10/08/2021_LCMS sample queue: Berezikov group, Stijn Mouton's samples

16/08/2021 Exosome-10 IP (new anti-FLAG beads, NaCl; 300 mM/700 mM, 150 mg cell powder) for SDS-PAGE analysis

16/08/2021 Exosome-10 IP (new anti-FLAG beads, NaCl; 300 mM and 700 mM, 150 mg cell powder) SDS-PAGE

16/08/2021_LCMS sample queue: Universal Protein Standard (300 mM NaCl IP samples, Apostolis and Nastya cell powder)

16/08/2021_LCMS sample queue: Arthur Flohr Svendsen samples

16/08/2021_Maxquant: Berezikov group, Stijn Mouton's samples

20/08/2021 Exosome-10 IP (new anti-FLAG beads, NaCl; 300 mM and 700 mM, 150 mg cell powder) NativePage Syphro ruby

26/08/2021 Exosome-10 IP (new anti-FLAG beads, NaCl; 300 mM/700 mM, 150 mg cell powder) dilution test folowed by native page analysis

02/09/2021 Exosome-10 IP (in gell digestion of native page bands

13/09/2021_Maxquant: Arthur Flohr Svendsen samples

14/09/2021 Exosome-10 IP (new anti-FLAG beads, NaCl; 300 mM/500 mM/700 mM, 150 mg cell powder) folowed by native page analysis

17/09/2021 Exosome-10 IP 300, 500 and 700 mM (in gell digestion of native page bands)

17/09/2021 Exosome-10 IP 300 mM new beads (in gell digestion of sds-page bands)

28/09/2021_S-Trap Micro Ulra High Recovery Protocol (300, 500 and 700 mM NaCl IP samples, for ZIPCHIP analysis)

08/10/2021 Mass Photometry (Human Her2 ErB2 Protein His tag, Baubek Spanov)

01/11/2021_S-Trap Micro Protocol (Berezikov Group, Joscha Muck)

02/11/2021_LCMS sample queue: Berezikov group, Joscha Muck's samples

04/11/2021 Exosome-10 IP (new anti-FLAG beads, NaCl; 700 mM, 150 mg cell powder) folowed by native page analysis and MP

04/11/2021 Mass Photometry (new anti-FLAG beads, NaCl; 700 mM, 150 mg cell powder, G-250)

09/11/2021 Exosome-10 IP (new anti-FLAG beads, NaCl; 700 mM, 150 mg cell powder) for MP, NativePAGE and SDS_PAGE analysis

09/11/2021 Mass Photometry (new anti-FLAG beads, NaCl; 700 mM, 150 mg cell powder, visit Refeyn)

16/11/2021_Maxquant: Berezikov group, Joscha Muck's samples

16/11/2021 SDS-PAGE (new anti-FLAG beads, NaCl; 700 mM, 150 mg cell powder, visit Refeyn)

03/12/2021 Mass Photometry (Human Her2 ErB2 Protein His tag, stress test, Baubek Spanov)

09/12/2021 Exosome-10 IP (new anti-FLAG beads, 100 mM MgCl2, 300 mM NaCl/5 mM Chaps, 150 mg cell powder) folowed by native page analysis and MP

09/12/2021 Mass Photometry (Exosome-10 IP, new anti-FLAG beads, 100 mM MgCl2, 300 mM NaCl/5 mM Chaps, 150 mg cell powder)

15/12/2021_microBCA protocol (Stijn Mouton samples)

29/12/2021_S-Trap Micro Ultra-High Recovery Protocol rev. 05/27/21 (Berezikov Group, Stijn Mouton, nuclei)

14/01/2022_LCMS sample queue: Berezikov group, Stijn Mouton's nuclei samples

06/02/2022_Maxquant: Berezikov group, Stijn Mouton's nuclei samples

DISCONTINUED DUE TO USING ANOTHER DOCUMENTATION SYSTEM