Cell lines

1. BG1 WT (no handle)
2. BG1 CDH1-HA
3. BG1 CDH1-HA TMTC2 KO (ZFN1048, clone D1)
4. BG1 CDH1-HA TMTC3 KO (ZFN1049, clone A9)
5. BG1 CDH1-HA TMTC2/3 KO (ZFN1050, clone D3)
6. BG1 CDH1-HA TMTC1-4 KO (ZFN1051, clone D8)
7. HEK293 6E WT (no handle)
8. HEK293 6E TMTC3-TEV-GFP (ZFN1090, clone 23)
9. HEK293 6E TMTC3-TEV-3xFLAG (ZFN1152, clone C6)

Materials

Reagent

1. HA-tagged Positive Control,
   • It is part of Pierce™ HA-Tag IP/Co-IP Kit, Thermo Scientific (Cat: 26180x, Lot:UD280691)
2. Anti-HA antibody
   • Invitrogen Anti-HA Tag Monoclonal (2-2.2.14), Catalog # 26183
   • ani-HA magnetic bead: https://www.thermofisher.com/order/catalog/product/88837

cell powders

the details of powders is stored at google driver and rockefeller ssh