plasmid design

1. The design of DNAJB1-PRKACA fusions is summarized here: Design of Dnajb1-Prkaca fusions - v3
   1. MTp483_tdTomato-P2A-PuroR gBlock here>
   2. MTp484_Dnajb1-Prkaca-3C-3xF gBlock here>
   3. MTp495_Guide1_gBlock here>
   4. MTp496_Guide2_gBlock here>
   5. pef37-px458optiv2.gb here>
   6. pMT877 copy of pef182 ucoe-efs-ns-mscarlet-2a-mneongreen-linker-3xflag-wpre here>
   7. pMT878 DnaJB1-PRKACA overexpression Lenti - v3 here>
   8. pMT879 DnaJB1-PRKACA dual guide Lenti v2 here>
   9. pMT880 DnaJB1-PRKACA dual guide Cas9 Lenti v2 here>

Native elution

Peptides

we synthesis 2 peptides from 21st century biochemicals Inc for native elution. the sequence is showed blew. and peptide certificate is stored here>

1. peptide1: c-KGPGDTSNFDDYEE-PEG4-KGPGDTSNFDDYEE-amide
2. peptide2: Ac-KGPGDTSNFDDYEE-amide