admin16th October 2021 at 1:33pm
Hela S3 FlipIN#11 Trex#3 WESTERN BLOT
TetR Antibody (NB600-234) Novus Biologicals
WB1:
Positive control: HEK FlipIN TREX (expresses tetR)
Hela S3 FlipIn#11 Trex#1 selected with Zeocin and Blasticidin
WB2: Positive control: HEK FlipIN TREX (expresses tetR)
Hela S3 FlipIn#11 Trex#1 selected with Zeocin and Blasticidin
I induced the HelaS3 FlipIn TREX to test if it influences tetR expression
New: tested few days after thawing cryo-vial Old: tested after cells were grown for longer time (~2weeks)
Western blot HelaS3 FlipIn#11 tREX#1,2,3 (all three clones)
