LaCava Research Wiki

Initiated September 2017

July 2021 - Raghu RC10_73

Luciano14th October 2021 at 12:09pm

Description: Sftpc-CreERT2 lung LysoIPs; to determine the resting proteome of the type II alveolar epithelial cell lysosome

Link to sample prep: https://macromolecule-child.rockefeller.edu/#13%2F11%2F2020_S-Trap%20Micro%20Protocol%20(Raghu%20Chivukula's%20LysoIP%20samples%2C%20RC10-30%2C%20changed%20protocol)

Resuspension solvent: 94.9:5.0:0.1 water:MeOH:FA

Resuspension volume: 25 uL

Injection volume: 1 uL

MS method: uPU-90mingradTOP20-highload_Luciano_375_1500

Link to RAW files: https://www.dropbox.com/sh/for6e8e8aa2blxv/AABM2hRKo0TDTyA-SXTApMrea?dl=0