Upon receiving, split each sample equally into 2 aliquots. One sent to
Kennedy C. Ukadike, MD, MS
Mustelin Lab, South Lake Union Campus
University of Washington School of Medicine
The other half, split into 5 aliquots and kept in the new -80C freezer in box "CDI recombinant proteins"
Also, run 250ng of each on a gel, followed by Sypro stain
Add LDS and 50mM DTT to each sample, heat @ 70C for 10’
Run 15-well Bis-Tris gel, Sypro stain; Loading order: • Unstained protein marker_1ul • Samples 1-13 • BSA_100ng (with 3ul of prestained marker in the same lane)
2-second exposure, auto tone
Sypro gel labeled with Molecular Mass of each protein
The recombinant proteins are human proteins expressed in yeast as a GST-tagged ectopic expression. So they will be ~26kDa heavier than the mass you find in Uniprot database.
