LaCava Research Wiki

Initiated September 2017

November 2020 - Raghu organs RC10_16_and_25

Luciano14th October 2021 at 12:00pm

Description: Lyso IP in mice organ in quadriplicates (lung, liver, brain) RCL25 are pooled samples that were s-trapped by Raghu for comparison. Brain IPs were ran using two different mass ranges (200-2000 and 375-1500). We concluded using this information that the range 375-1500 gives more IDs.

Link to sample prep: https://macromolecule-child.rockefeller.edu/#13%2F11%2F2020_S-Trap%20Micro%20Protocol%20(Raghu%20Chivukula's%20LysoIP%20samples%2C%20RC10-30%2C%20changed%20protocol)

Resuspension solvent: 94.9:5.0:0.1 water:MeOH:FA

Resuspension volume: 25 uL

Injection volume: 1 uL

MS method: uPU-90mingradTOP20-highload_Luciano and uPU-60mingradTOP20-highload_Luciano_375_1500

Link to RAW files: https://www.dropbox.com/sh/ca9v2rr4pkswjsa/AAD1waMy3oCnW4VDQhH7AijYa?dl=0