LaCava Research Wiki

Initiated September 2017

October 2020 - Hua samples ORF1 using different cell lines direct IPs

admin9th January 2022 at 2:05pm

Description: antiORF1 IPs on N2102Ep, NTERA, PA1-PC

Link to experiment: September 29, 2020 - L1 endogenous cell line anti-ORF1 and mIgG for MS

Link to sample prep (handled by Hua): MS sample preparation using S-Trap Micro Ultra-High Recovery protocol

Resuspension solvent: 94.9:5.0:0.1 water:MeOH:FA

Resuspension volume: 20 uL

Injection volume: 5 uL as indicated in the Raw file’s name

MS method: uPU-60mingradTOP20-highload_Luciano_IP_gradient (for DDA) uPU-60mingradTOP20-Hload_DIA_300_1500_window_15 (for DIA)

Link to RAW files: https://www.dropbox.com/sh/kgivt99h80e96in/AABbgdpNsqYM0_UHOLnFcoOba?dl=0