LaCava Research Wiki

Initiated September 2017

PA-1 PC culture for getting BBs

Maria10th January 2020 at 11:17am

Seeded (07-03-18) 6 flask T150 -> pass them to 16 dishes (07-06-18) Harvest (07-09-18) -> yield: around 3-3.5 gr of BBs

Seeded (07-06-18) 4 flasks T150 -> pass them to 16 dishes (07-10-18). Change the media (07-13-18). Cells were harvested the (07-16-18) yield: around 3-3.5 of BBs

Both harvested cells (from 07-09-18 and 07-13-18) were mixed and cryomilled together. We obtained 5.85 gr of powder.

Seeded (07/26/19) 6 flask T150 -> pass them to 16 dishes (07/29/19) (80 ml of media each dish - 1280 ml of media)