LaCava Research Wiki

Initiated September 2017

Ribosomal subunit binding of ORF1p

Lars 18th August 2020 at 6:13am

I first did a tester just before the lockdown: 1. Tester for ribosomal subunit binding of ORF1p_20200316

Then Hua repeated the experiment just with MT302. These samples were however analyzed on the nano chip. –> August 5, 2020 - anti-ORF1 IP with EDTA or MgCl2

We also realized we need less salt to properly look at RNA interactions. Experiment will be repeated once more with 150mM NaCl instead of 500mM.