LaCava Research Wiki

Description: antiORF1 IPs on N2102Ep, NTERA, PA1-PC and PA1-DC proteins after RNA extraction obtained from organic phase.

Link to experiment: https://macromolecule-child.rockefeller.edu/#September%2010-17%2C%202020%20-%20RNA%20extraction%20and%20MS%20sample%20prep%20in%20endogenous%20cell%20lines%20following%20mock%20IP%20with%20mIgG

Link to sample prep (handled by Hua): https://macromolecule-child.rockefeller.edu/#MS%20sample%20preparation%20using%20S-Trap%20Micro%20Ultra-High%20Recovery%20protocol

Resuspension solvent: 94.9:5.0:0.1 water:MeOH:FA

Resuspension volume: 20 uL

Injection volume: 1, 2 or 5 uL as indicated in the Raw file’s name

MS method: uPU-60mingradTOP20-highload_Luciano_IP_gradient

Link to RAW files: https://www.dropbox.com/sh/kgivt99h80e96in/AABbgdpNsqYM0_UHOLnFcoOba?dl=0