50 mg of powder - Extract 1:4 with extraction buffer – sonicate and collect the cleared lysate
Bradford with SUP (to check protein concentration)
BCA to quantify protein concentration for Wes (accurate)
Wes to test antibodies:
ORF1p, ORF2p, OCT4 and FOXF2
Wes didn't work. We couldn't see any specific bands around the desired MW.