By default, when running TiddlyWiki on Node.js, the server exposes the wiki at the URI formed from the protocol, host and port - for example, http://127.0.0.1:8080/.
There are two steps to running the wiki at a custom path like http://127.0.0.1:8080/path/to/my/wiki/:
/path/to/my/wiki as the pathprefix argument of the ServerCommand$:/config/tiddlyweb/host that contains $protocol$//$host$/path/to/my/wiki/