—> confirmed in HEK by IP/MS; western blot results for elution fractions are ambiguous for HeLa / CRC (might not be expressed, needs more investigation) - no expression detected in lysates (sup, pel or ft). 24 well screen was carried out, but suboptimal sonication was used. LFQ MS done at 150 and 300 mM NaCl. Preliminary Glutaraldehyde treatment test suggests antibody works on crosslinked material (IPs target). This no expression statement - are you referring only to HeLa? Because we have detected TP53 in the lysates for HEK cells.
—> Need side-by-side PO +/- Iodacetamide to try and resolve "fuzzy" bands issue