(alphabetical)
NFKB1-3xFLAG –> cell line made
STAT3-3xFLAG —> cell line made
SMAD4-3xFLAG —> cell line made
TP53-3xFLAG —> amino acid substitutions, needs MISO (in progress)
3xFLAG cell lines made
STAT3-3xFLAG —> Leaky expression. Does not appear to purify a complex BUT needs to be tried under TP53 sonication conditions (25x2s) and/or w/ glutaraldehyde. We can compare performance of CDI anti-STAT3 to anti-FLAG now (done, see here )
SMAD4-3xFLAG —> Expression looks ok. Other results pending.