Given the dubious sample handling at the genomics core facility at RU, we have looked at alternatives and are now allowed to make use of the BioAnalyzer of the Jarvis lab, all the way at the end on 2 North of the KRB.
MATERIALS
| Name | Company | Cat. nr. | Date received/made | Comments | ||||
|---|---|---|---|---|---|---|---|---|
| RNA pico 6000 kit | Agilent | 5067-1513 | ||||||
| RNA pico 6000 chips | Agilent | Stored above Lars' bench | ||||||
| Pico Dye Concentrate | Agilent | Stored 4°, 2nd refrigerator, ‘Bioanalyzer reagents’ box | ||||||
| Pico Marker | Agilent | Stored 4°, 2nd refrigerator, ‘Bioanalyzer reagents’ box | ||||||
| Pico Conditioning Solution | Stored 4°, 2nd refrigerator, ‘Bioanalyzer reagents’ box | |||||||
| Pico Gel Matrix | Agilent | Stored 4°, 2nd refrigerator, ‘Bioanalyzer reagents’ box | ||||||
| RNA 6000 Pico Ladder | Agilent | Aliquots stored at -80, top left drawer, 'Qubit reagets' box | ||||||
| Spin Filters | Agilent | Stored 4°, 2nd refrigerator, ‘Bioanalyzer reagents’ box, extra above Lars' bench |
PROTOCOL
Preparing the chips is the most work, running is straightforward. See below