LaCava Research Wiki

Here, routine / established protocols should be cataloged. Link to these protocols within experimental entries. When changes are made within otherwise routine procedures, date stamp the change within the text, or, for significant changes, create a new entry with a sequential version number.

Cell Culture Protocols

Counting Cells

Luna-FL automated cell counter

MS sample preparation protocol using OMIX C18 tips for gel bands

MS sample preparation protocol using OMIX C18 tips for gel plugs

MS sample preparation protocol using Stage Tips for gel plugs

MS sample preparation using S-Trap Micro Protocol rev 10/25/20

MS sample preparation using S-Trap Micro Ultra-High Recovery protocol

MS sample preparation using S-Trap Micro Ultra-High Recovery protocol rev. 12/22/20

MS sample preparation using S-Trap Micro Ultra-High Recovery protocol rev. 05/27/21

Conjugation of antibody Dynabeads

Conjugation of abmart 4H1 anti-ORF1 Dynabeads

Immunoprecipitation with antibody conjugated Dynabeads from cell powder

RNA extraction after cell powder IP

RNA extraction after cell powder IP with on column DNase treatment

Infors HT Multitron – How to use + manuals

Initial Tests for Tagged Protein Affinity Capture (from Docollab)