LaCava Research Wiki

Initiated September 2017

Leila Saba's Journal

admin25th January 2022 at 7:54am

12 January 22_anti-ORF2 Bead Conjugation and Testing

4 January 22_Large Scale N2102EP and NTERA IPs for PacBio sequencing

30 August 2021 N2102EP Suspension Culture Trial

24 June 21_Organoid Cell Culture Training

5 June 21_Enhanced Protocol BCA Assay of worm nucleii for Stijn (Berezkhov Lab)

4 June 21_BCA Assay of worm nucleii for Stijn (Berezkhov Lab)

28 May 21_ROME N2102EP Drug Assay anti-ORF1 IP

26 May 21_N2102EP Drug Assay Prep_Dynabeads Testing

17 May 21_N2102EP Drug Assay Cell Growth, Drug Dosage and Harvest

23 April 21_N2102EP Drug Assay Prep_Pilot Test of Media Volume Increase

21 October 20_Dynabeads Final Testing

01 October 20_Optimize N2102EP for Wes/Jess

29 September 20_New Dynabeads Testing

18 August 2020_Bead testing Leila/Lars

30 June 2020_Triplicate test for ZCCHC8

24 June 2020_Test for ZCCHC8 Hua's and Leila's powder

23 June 2020_Preliminary Test for ZCCHC8

04 June 2020_EXOSC10 purification in NaCl 300mM and 700mM

12 May 2020_Optimize sonication conditions for QSonica Q700

20 February 2020_Triplicate RRP6 IP

23 September 2019_anti-FLAG bead conjugation

20 August 2019_anti-GFP bead conjugation

15/03/2019_PVSA Testing in 162A and MT302

18/02/2019 - 21/02/2019_Sabatini Lab Suspension Culture Training

23/01/2019_162T RNA Preparation - Zymo and Phaselock

18/01/2019_RNA FOR Zbig

15/01/2019_L1 IP RNA Preparation – Zymo MicroPrep kit

07/01/2019_L1 IP RNA Prep - Qiagen vs Zymo in MT302 and PA-1

30/10/2018_Duplicate anti-ORF1 PO in 144T

10/16/2018_Triplicate anti-ORF1 PO in 144T

10/09/2018_144T anti-ORF1 Bead Titration Test (repeat)

09/28/2018_144T anti-ORF1 Bead Titration Test

09/25/2018_Extract RNA from CRC Tissue, Trizol extraction and parallel anti-ORF1 IP

09/12/2018_144T and 157T Test IP with ORF1_pretest

08/20/2018_RNA Tumor IP Test with ORF1

08/13/2018_Extract RNA from CRC tissue, anti-ORF1 IP

08/01/2018_Extract RNA from Human Tissues (optimize tissue RNA prep conditions)

07/13/2018_Extract RNA from Tumor Tissues (optimize tissue RNA prep conditions)

07/07/2018_Extract RNA from Human Tissue in Trizol

07/06/2018_Testing Gibco vs. Sigma FBS

06/21/2018_Extract RNA from anti-ORF1 IP

06/15/2018_Bead Wash Test - BSA removal

05/29/2018_Extract RNA from anti-ORF1 IP

05/10/2018_Testing 16-pin sonicator arm

24-well Screen Sonication Test on Wes (repeat 05/02/18)

03/28/2018_16-well Sonication Test on Wes

03/18/2018_CDI Protein Standard Test with anti-GST

03/12/2018_24-well Screen Sonication Test on Wes

02/13/2018_TP53-3xFLAG Test PO on 8% Tris-Glycine gel

02/08/2018_Repeat PO TP53-3xFLAG_antiTP53 vs anti-FLAG Wes vs WB

01/31/2018_TP53-3xFLAG_anti-TP53 vs anti-FLAG PO

TP53-3xFLAG Test PO using anti-FLAG beads

TP53-3xFLAG_Cell Line Induction and Dox Test

Extractman Test PO (repeat) 10/20/2017

TP53 Gibson Assembly

NFkB1-3xFLAG Cell Line Test

STAT3-3xFLAG PO: anti-FLAG vs anti-STAT3 beads

TF 3xFLAG Flp-In constructs

06/07/2017_Quadruplicate anti-TP53 PO for MS in HEK293

06/19/2017_Quadruplicate anti-STAT3 PO for MS in HEK293 and HeLa

FINAL MISO PCR for TP53

Testing of new CDI Antibodies