February 3, 2022 - Making and testing new anti-ORF2 (9-7 and 5-5) beads
February 2, 2022 - Anti-ORF2 IP (MT646, MT647, LD564)
October 21, 2021 - MT646 S9.6 Tandem IP with RNase treatment before final elution
October 14, 2021 - MT646 and MT302 Tandem IP with anti-ORF1 and S9.6 beads
October 5, 2021 - Effect of RNase treatment on RNA/DNA hybrid and L1 RNP (MT646)
September 29, 2021 - anti-FLAG IP with MT646, MT647, LD564, MT302 and LD401
September 23, 2021 - Checking L1 expression of MT646, MT647 and LD564
September 8-15, 2021 - Conjugation of anti-ORF1 Dynabeads and attempt to reduce IgG leakage
September 2, 2021 - Concentrating and buffer exchange of anti-ORF1 (4H1)
August 30, 2021 - Testing Thrombin Sepharose Beads and Glutathione Resin
August 27, 2021 - Testing new MT302 powder
August 11-27, 2021- Transfection of HEK293TLD in suspension using PEI Max
August 18, 2021 - Testing new anti-ORF2 (clone 9-7)
August 16, 2021 - Making and testing anti-ORF1, anti-GFP and anti-FLAG beads
July 2021 - CRC 24-well screen_ 162 and 197
June 28, 2021 - Testing sonication conditions for 50mg powder
June 24, 2021 - CRC-197 anti-ORF1 IP with controls and beads titration
June 24, 2021 - Checking L1 expression of 9 L1 constructs
June 16, 2021 - pMT646 and pMT647 anti-FLAG IP
June 14, 2021 - Anti-ORF1 IP with senescent cells
June 9, 2021 - LINE-1 tandem prep with or without LaORF1 for EM
June 3, 2021 - Testing new anti-ORF2 beads and ORF2 dipeptides_2nd round
May 27, 2021 - Testing new anti-ORF2 beads and ORF2 dipeptides
May 27, 2021 - Testing new anti-ORF1 and mIgG beads
May 25, 2021 - Conjugation of anti-ORF1, anti-ORF2 and mIgG Dynabeads
May 24, 2021 - Anti-ORF1 IP with senescent cells
May 18, 2021 - SLE Neutrophils anti-ORF1 Western
May 14, 2021 - RRP6 purification followed by dialysis
May 13, 2021 - Effect of ZnCl2 and hUdT on CRC RNA quality
May 10, 2021 - Effects of ZnCl2 and hUdT on IP specificity and RNA quality
May 5, 2021 - Effects of ZnCl2 on IP specificity using CRC tissue
May 4, 2021 - Effects of ZnCl2 on IP specificity using different cell lines and tissue
May 3, 2021 - LD401 mIgG IP testing effects of ZnCl2 and new RNase inhibitor on IP specificity
April 30, 2021- N2102Ep anti-ORF1 IP with new RNase inhibitor and mIgG control
April 29, 2021- N2102Ep anti-ORF1 IP with or without new RNase inhibitor
April 27, 2021- RNA prep from CRC with new RNase inhibitor
April 19, 2021 - RNA prep with 3'-N-hydroxyurea-3'- deoxythymidine 5'-monophosphate
April 16, 2021 - Check SLE Western samples from Seattle
April 12, 2021 - Testing RRP6 powder
April 8, 2020 - Making and testing new anti-ORF1 beads
April 5, 2021 - Making and testing S9.6 and anti-ORF1 beads
March 30, 2021 - MT289, N2102Ep Tandem PO with S9.6 beads
March 24, 2021 - LINE-1 tandem prep_with or without LaORF1 for EM
March 22, 2021 - N2102Ep Tandem PO with S9.6 beads
March 9, 2021 - EXOSC10 (RRP6) gradient for EM
March 2, 2021 - N2102EP L1 screen in 24-well format (4x 6 conditions)
February 24, 2021 - Making and testing of mouse and rabbit IgG Dynabeads
February 17, 2021 - L1 RNP preps to capture human auto-antibodies from serum/plasma_2nd test
February 16, 2021 - Nucleic acids characterization in L1 RNPs_Collaboration with John Sedivy
February 10, 2021 - LD401 Tandem IP for EM_test ORF1 nanobody binding
January 28, 2021 - L1 RNP preps to capture human auto-antibodies from serum/plasma
January 7, 2021 - Large scale N2102Ep, NTERA anti-ORF1 and anti-ORF2 IPs for PacBio Sequencing
December 15, 2020 - N2102Ep, NTERA and CRC anti-ORF1 and anti-ORF2 IPs with controls
December 3, 2020 - N2102EP L1 screen in 24-well format
November 19, 2020 - Test ORF1 and ORF2 peptide for native elution after RNase A/T1 treatment
November 17, 2020 - N2102Ep anti-ORF1 IP with Nuclease treatments
November 10, 2020 - LD401 Tandem IP for EM with RNase A/T1 treatment on anti-FLAG beads
November 5, 2020 - RRP6 purification with BSA incubation
October 29, 2020 - Checking extraction conditions for 197N and 268N
October 28, 2020- RNA prep from CRC with DNase I treatment
October 22, 2020- RNA prep from CRC with and without EDTA
October 20, 2020 - N2102Ep and NTERA anti-ORF2 IP for Western
October 12, 2020 - N2102Ep anti-ORF1 and anti-ORF2 IPs
October 7, 2020- L1 endogenous cell line anti-ORF2 and rIgG IPs
October 5, 2020 - Summary of materials for CRC project
September 30, 2020 - LD401 Tandem for EM
September 29, 2020 - L1 endogenous cell line anti-ORF1 and mIgG for MS
September 23, 2020 - RRP6 purification with RNase A/T1 treatment for EM_092320
September 22, 2020 - Making and testing anti-ORF1 beads
September 17, 2020 - RRP6 purification with 300mM and 500mM buffer
September 16, 2020 - LD401 Tandem IP for EM
September 9, 2020 - RRP6 purification for EM
September 2-9, 2020 - Making and testing anti-FLAG beads with antibody from Marty
August 2020 - Removing 3x FLAG peptide from native elution
August 19, 2020 - RRP6 purification for EM
August 5, 2020 - anti-ORF1 IP with EDTA or MgCl2
July 30, 2020 - Optimizing AmAc concentration for protein precipitation
July 28, 2020 - Anti-ORF1 IP with N2102Ep and NTERA2
July 22, 2020 - CDI recombinant proteins
July 21, 2020 - AmAc/MeOH precipitation of protein from anti-ORF1 elution in organic phase
July 15, 2020 - Test Experiment of AmAc/MeOH precipitation_4th trial
July 7, 2020 - Testing anti-FLAG and uncoupled beads from ERIBA
July 6, 2020 - Anti-ORF1 IP with N2102Ep and NTERA2_070820
June 30, 2020 - Checking LaORF1 and LaG94 beads before and after DMP XL
June 29, 2020 - anti-ORF1 IP using anti-LaORF1 beads
June 29, 2020 - Test Experiment of AmAc/MeOH precipitation_3nd trial
June 22, 2020 - Test Experiment of AmAc/MeOH precipitation_2nd trial
June 18, 2020 - Test Experiment of AmAc/MeOH precipitation
June 17, 2020- CRC (159_185_174) anti-ORF1 IP
June 16, 2020 - Western Blots of Lars' samples
June 10, 2020 - CRC (162_185_197_197_268_284) anti-ORF1 IP
June 9, 2020 - Summary of anti-ORF1p RNA extractions by Lars
June 9, 2020 - Update on CRC project
Mar. 2, 2020 - SEC of ORF2 mAb (9-7)
Feb. 26, 2020 - Testing anti-ORF2 beads
Feb. 19, 2020 - SEC of ORF2 mAb (9-7)
Feb. 12, 2020 - Testing anti-ORF1_anti-ORF2 beads and RRP6-3xFLAG powder
Jan. 29, 2020 - RRP6 purification
Jan. 22, 2020 - Capturing ORF2p with anti-ORF2 beads from N2102Ep
Jan. 7, 2020 - Capturing ORF2 with rabbit anti-ORF2 beads from N2102Ep and MT302
12/19/19 - PA-1, N2102Ep and NTERA2 anti-ORF1 IP
12/11/19 - Reuse anti-ORF1 for beads conjugation
12/04/19 - anti-FLAG, anti-ORF1 and anti-ORF2 beads titration test
11/12/19 - Making and testing LaORF2-12 and LaORF2-16 beads
11/06/19 - Making and testing LaORF2-12 beads
10/29/19 - Making and testing LaORF2 beads nanobodies
10/23/19 - Making and testing LaORF1 and LaG94 beads
10/22/19 - Making and testing anti-ORF1 and mIgG beads
10/17/19 - Antibody recovery from antibody mix after beads coupling
10/08/19 - anti-FLAG and anti-ORF2 beads titration test
10/07/19 - Conjugation of rabbit IgG Dynabeads
10/01/19 - Checking and recovery of anti-ORF2 (abcam, clone 9) antibody
09/30/19 - MT302 PO testing anti-ORF2 beads with or without XL
09/26/19 - Making_Testing anti-ORF2 beads
09/19/19 - Testing anti_ORF2 and LaORF2 beads
09/18/19 - Making anti-ORF2 (abcam Clone 9-7) beads with increasing AmSO4 concentration
09/16/19 - Making and testing abcam anti-ORF2 beads
09/14/19 - Testing abcam anti-ORF2 for Western
09/12/19 - abcam anti-ORF2 antibody info
09/09/19 - RNVC Testing – RNA Prep from MT302 and 162T
09/03/19 - 162T and 162N IP using PrG or PrA beads
08/26/19 - Making and testing anti-ORF1 beads
08/07/19 - Anti-ORF1 IP using PA-1 PC cells with crosslinked LaORF1 beads
08/05/19 - Orf2 (MT302) PO testing LaORF1 beads with or without XL
07/31/19 - Conjugation of LaORF1-5, LaG94-10 and anti-FLAG Beads
07/26/19 - BSA Standards in Urea and LDS
07/18/19 - Conjugation and testing anti-FLAG beads
07/10/19 - LD401_anti-FLAG and anti-ORF1 beads titration
07/08/19 - Conjugation of anti-ORF1 Dynabeads
07/02/19 - Prepare Whole Cell Samples Using S-Trap Micro Spin Column Digestion Protocol
06/28/19 - Sample Prep for PROTIFI
06/25/19 - Prepare IP Elution for MS Using S-Trap Micro Spin Column Digestion Protocol
06/17/19 - Testing new Abmart anti-ORF1 by Western
06/11/19 - LD567 Large Scale IP
06/07/19 - Making and testing anti-FLAG and anti-ORF1 beads
06/04/19 - Making and testing anti-FLAG and anti-ORF1 beads
05/28/19 - 162T and 162N anti-ORF1 IP_Sonication_Beads titration_Time course
05/16/19 - LD401, LD561 and pCEP-Puro anti-FLAG IP_400mg Scale
05/13/19 - LD401, LD561 and pCEP-Puro anti-FLAG IP
05/09/19 - L1 expression in LD401, MT302 and MT289
05/06/19 - ORF2/ORF1 Tandem to check LD401 powder
04/15/19 - MT302 and LD288 PO testing Llama anti-ORF2 antibody
04/08/19 - LD288, LD603 and pCEP-Puro anti-FLAG IP
04/03/19 - anti-ORF1 IP with MT302, PA1, HEK and HeLa cell lines
03/20/19- LD561 PO for MS using Gel Plugs or S-Trap
03/19/19 - Conjugating and testing anti-ORF1 beads
03/15/19 - L1 expression in MT289, MT302 and LD401 BB’s from Marty
03/13/19 - NCBP3 IP with GA Crosslinking
03/08/19 - CBP80 (NCBP1) anti-GFP IP_50mg scale
03/07/19- Conjugating Dynabeads with Llama anti-GFP antibody
03/06/19 - L1 expression in new MT302, LD401 and MT289 powder
02/27/19 - PA-1 and MT302 purification for MS
02/22/19 - Orf2 (MT302, PA-1) Test PO using anti-ORF2 Protein G beads
02/21/19 - Cross-linking of anti-ORF2 to Protein G beads
01/31/19 - Endogenous L1 Element Amplification (eLEAP) assay on MT302 and 162T / N
01/29/19 - eLEAP assay on PA-1 (50 and 100mg scale) with heat inactivation
01/14/19 - Endogenous L1 Element Amplification (eLEAP) assay on PA-1 and MT302
01/09/19 - anti-ORF1 IP_Native Elution with ORF1 dipeptide
11/27/18 - Pullout with Ring 1B or mouse IgG beads using estrogen induced cells
11/19/18 - 162T and 164N anti-ORF1 PO for Western (Elution only)
11/16/18 - ORF2 (MT302) PO for EM_Urea
11/14/18 - 162T and 162N anti-ORF1 PO and Western of Lysate
11/12/18 - Isotope check for MT302H, MT289L and 302H/289LMix_SDS
11/07/18 - LD561 Purification for EM with Nucleases treatment
10/31/18 - ORF2 (MT302) test PO with different buffer conditions
10/30/18 - ORF2 (LD561) test PO with different buffer conditons
10/29/18 - Conjugating anti-Ring1B
10/24/18 - ORF2 (LD561)_PO for EM_with Nuclease treatment
10/18/18 - LD561 Test PO with Nucleases (Benzonase, RNase A/T1) treatment
10/17/18 - Isotope check MT302H, MT289L, 302H/289L mix
10/16/18 - ORF2 (LD561)_Test PO with Benzonase treatment
10/09/18- Anti-ORF1 IP with MT302 and PA-1 PC cells: titration of LaORF1 beads
10/03/18 - RRP6 purification for EM_Effect of detergent concentration of elution
09/27/18 - LINE1 glutaraldehyde crosslinking
09/24/18 - MT302 and PA-1 PC PO with anti-ORF1 beads
09/21/18 - anti-ORF1 beads survey
09/20/18 - Orf2 (MT302) PO with ORF1 beads titration
09/12/18 - Orf2 (MT302) PO with anti-ORF1 beads with or without XL
09/06/18 - RRP6 purification and concentration
09/04/18 - Orf2(MT302) Purification followed by RNAse treatment
08/29/18 - Orf2(MT302) Purification followed by RNAse treatment
08/23/18 - LD288 IP testing anti-FLAG beads
08/22/18 - RRP6 purification followed by gradient for EM
08/17/18 - RRP6 Purification with Native Elution
08/03/18 - Orf2 (MT302) Tandem IP using anti-FLAG and m6A beads with RNases treatment
08/02/18- Making anti-m6A Protein G beads_080218
07/31/18 - RRP6 Purification: Adhesive vs Suspension Cells
07/25/18 - LD288 anti-ORF1 PO (Epoxy beads without XL)
07/24/18 - anti-LaORF1 beads conjugation (Epoxy beads) with incresing amount of nanobody or AmSO4
07/18/18 - ORF2 (LD401) and RRP6 anti-FLAG PO
07/16/18 - Cross-linking (XL) of LaORF1 to Protein G or Protein A beads
07/13/18 - LD288 anti-ORF1 PO (Epoxy beads without XL)
07/12/18 - anti-LaORF1 beads conjugation (Epoxy beads)
07/11/18 - RNA Methylation in MT302 and LD401 Total RNA from Native Eluates
07/09/18 - RNA Methylation in MT302 and LD401 Native Eluates
06/29/18 - BSA Standard Stock for Gel
06/27/18 - RNA Methylation Quantification (MT302)
06/20/18 - Isotope check: LD401H and LD401H/LD259L
06/19/18 - Anti-ORF2 Protein G beads
06/18/18 - Orf2 (LD401, MT302) PO testing antibodies
06/13/18 - Effect of RNase treatment on RNA/DNA hybrid and Orf2 elution
06/12/18 - Effect of RNase H treatment on RNA/DNA hybrid
06/07/18 - Orf2 (MT302) Tandem IP using anti-FLAG and S9.6 beads with RNases treatment
06/05/18 - Orf2 (MT302) Tandem IP using anti-FLAG and S9.6 beads
05/31/18 - Orf2 (LD401) Tandem PO with S9.6 beads
05/30/18 - Anti-ORF2, S9.6, rIgG, mIgG Protein G beads
05/21/18 - Anti-ORF1 and anti-ORF2 Western in Human CRC tissues
05/15/18 - Testing S9.6 Protein G beads with Fluorescent Labeled Oligos and RNase treatment
05/14/18 - Testing S9.6 beads with fluroscent labeled oligos
05/09/18 - Testing S9.6 Antibody using LD401 Native Elution and Fluorscent Oligo
05/02/18 - Cross-linking of anti-DNA-RNA hybrid antibody (S9.6) to Protein G beads
05/01/18 - Test PO with anti-UPF1, NCBP1 and PABPC4 Protein G beads in HEK293 cells
04/30/18 - Cross-linking (XL) of anti-UPF1, NCBP1 and PABPC4 to Protein G beads
04/26/18 - Cross-linking (XL) of anti-ORF2 to Protein G beads
04/20/18 - Orf2 (LD401) Test PO using anti-ORF2 Protein G beads
04/19/18 - Cross-linking (XL) of anti-ORF2 to Protein G beads
02/12/18 - Testing S9.6 antibody against RNA/DNA hybrid
02/05/18 - MT302Heavy/MT289Light_I-DIRT 24-well Screen
02/01/18 - MT302Heavy/MT289Light_I-DIRT Manual Screen
01/24/18 - LD401Heavy/LD259Light mix_I-DIRT Screen
01/23/18 - Orf2 (MT302) PO with different amount of 3xFLAG peptide
01/19/18 - Orf2 (MT302) PO with different amounts of beads and peptide
01/17/18 - Orf2 (MT302) PO with different amount of anti-FLAG beads
01/12/18 - Orf2 expression in LD401 and MT302
01/12/18 - Orf2 (LD401) PO with post-PO Nuclease treatment
01/05/18 - Isotope check for L1 I-DIRT
01/03/18 - Protein Assay of L1 Heavy and Light Powder
12/07/17 - anti-KLF5 and anti-RELA PO and Western in HEK and HeLa
12/06/17 - Conjugating Dynabeads with CDI mouse mAbs
12/04/17 - anti-DACH1 PO and Western
11/16/17 - anti-FLAG and anti-NFKB1 Western
11/09/17 - NFKB1-3xFLAG test PO
11/07/17 - STAT3-3xFLAG Large Scale PO_150mM NaCl with control
10/31/17 - STAT3-3xFLAG Large Scale Purification_300mM NaCl
10/26/17 - LD401Heavy/LD259Light mix_I-DIRT 24-well Screen
10/16/17 - ORF2 (LD567 and LD624) anti-FLAG IP for RNA-seq
10/12/17 - ORF2/ORF1 Tandem for RNA-seq
10/05/17 - Total RNA prep with or without RNasin (LD401)
09/22/17 - ORF2 (LD567 and LD624) anti-FLAG IP for RNA-seq
09/20/17 - Making new anti-FLAG beads and test PO (LD401, 567, 624)